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pwpi lentiviral expression vector  (Addgene inc)


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    Structured Review

    Addgene inc pwpi lentiviral expression vector
    Pwpi Lentiviral Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 220 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pwpi+lentiviral+expression+vector/pWPI+(Plasmid+%2312254)/pmc11208297__BLOOD_BLD___2023___022655___mmc1-18-17-21
    Average 95 stars, based on 220 article reviews
    pwpi lentiviral expression vector - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Amplification:

    Article Title: CDK6 is activated by the atypical cyclin I to promote E2F-mediated gene expression and cancer cell proliferation.
    Article Snippet: .. Human cDNA of CCNI and CCND1 were amplified from cells with a C-terminal Flag tag and cloned into pWPI lentiviral expression vector (#12254; Addgene, Watertown, MA, USA). ..

    Article Title: Select human cancer mutants of NRMT1 alter its catalytic activity and decrease N-terminal trimethylation.
    Article Snippet: .. Wild type (WT), N209I, or P211S human NRMT1 were amplified from the pet15b vector to introduce a 5’ PmeI restriction site and a 3’ PmeI restriction site, and subcloned into pWPI lentiviral expression vector (Addgene). .. GFP-tagged lentivirus expressing WT NRMT1, N209I NRMT1, or P211S NRMT1 were made by co-transfecting HEK293LT cells with 50 µg pWPI containing the appropriate NRMT1 cDNA, 37.5 µg psPAX2 packaging vector, and 15 µg pMD2.G envelope plasmid using calcium phosphate transfection.

    Article Title: CDK6 is activated by the atypical cyclin I to promote E2F ‐mediated gene expression and cancer cell proliferation
    Article Snippet: .. Human cDNA of CCNI and CCND1 were amplified from cells with a C‐terminal Flag tag and cloned into pWPI lentiviral expression vector (#12254; Addgene, Watertown, MA, USA). ..

    Article Title: Select human cancer mutants of NRMT1 alter its catalytic activity and decrease N‐terminal trimethylation
    Article Snippet: .. Wild type (WT), N209I, or P211S human NRMT1 were amplified from the pet15b vector to introduce a 5′ PmeI restriction site and a 3′ PmeI restriction site, and subcloned into pWPI lentiviral expression vector (Addgene). .. GFP‐tagged lentivirus expressing WT NRMT1, N209I NRMT1, or P211S NRMT1 were made by cotransfecting HEK293LT cells with 50 μg pWPI containing the appropriate NRMT1 cDNA, 37.5 μg psPAX2 packaging vector, and 15 μg pMD2.G envelope plasmid using calcium phosphate transfection.

    Article Title: N-terminal acetylation and methylation differentially affect the function of MYL9
    Article Snippet: .. To generate C-terminally tagged MYL9-FLAG proteins, WT, SPK, and SSQ MYL9 were amplified from the MYL9-pDendra2 constructs and subcloned into the pWPI lentiviral expression vector (Addgene, Cambridge, MA) using 5′ and 3′ PmeI restriction sites. .. The following 3′ primer included the FLAG nucleotide sequence followed by a stop codon: 5′-CGGTTTAAACTCATTTATCATCATCATCTTTATAATCGTCGTCTTT ATCCTTGGCGC-3′ Lentivirus was generated through co-transfecting HEK293T cells with 50 μg of expression plasmid (pCDH or pWPI from above), 37.5 μg psPAX2 packaging vector, and 15 μg pMD2.G envelope plasmid using calcium phosphate transfection as previously described [ 47 ].

    FLAG-tag:

    Article Title: CDK6 is activated by the atypical cyclin I to promote E2F-mediated gene expression and cancer cell proliferation.
    Article Snippet: .. Human cDNA of CCNI and CCND1 were amplified from cells with a C-terminal Flag tag and cloned into pWPI lentiviral expression vector (#12254; Addgene, Watertown, MA, USA). ..

    Article Title: CDK6 is activated by the atypical cyclin I to promote E2F ‐mediated gene expression and cancer cell proliferation
    Article Snippet: .. Human cDNA of CCNI and CCND1 were amplified from cells with a C‐terminal Flag tag and cloned into pWPI lentiviral expression vector (#12254; Addgene, Watertown, MA, USA). ..

    Clone Assay:

    Article Title: CDK6 is activated by the atypical cyclin I to promote E2F-mediated gene expression and cancer cell proliferation.
    Article Snippet: .. Human cDNA of CCNI and CCND1 were amplified from cells with a C-terminal Flag tag and cloned into pWPI lentiviral expression vector (#12254; Addgene, Watertown, MA, USA). ..

    Article Title: CDK6 is activated by the atypical cyclin I to promote E2F ‐mediated gene expression and cancer cell proliferation
    Article Snippet: .. Human cDNA of CCNI and CCND1 were amplified from cells with a C‐terminal Flag tag and cloned into pWPI lentiviral expression vector (#12254; Addgene, Watertown, MA, USA). ..

    Article Title: A systematic analysis of orphan cyclins reveals CNTD2 as a new oncogenic driver in lung cancer
    Article Snippet: .. All the sequences were cloned into pWPI lentiviral expression vector (#12254, Addgene) at the Pme I restriction site. .. For lentivirus production, 45 μg of lentiviral expression vector were cotransfected with 12.9 μg of pMD2.G and 29.1 μg of psPAX2 into HEK293-T cells in 10 cm plates using calcium phosphate.

    Article Title: A lineage-specific STAT5B N642H mouse model to study NK-cell leukemia
    Article Snippet: Parental and transduced KHYG-14 (purchased from DSMZ) and IMC-15 (kindly provided by Prof. Chen, University of New Mexico Comprehensive Cancer Center) human NK-cell lines were cultured with RPMI complete with 100 U/ml, 25 U/ml or without human IL-2 (Proleukin, SteriMax). .. Lentiviral transduction of human NK-cell lines Human non-mutant (WT) STAT5B or STAT5BN642H transgenes were cloned into a pWPI lentiviral expression vector (Addgene plasmid # 12254; kindly provided by the lab of Prof. Hoermann, previously at the Department of Laboratory Medicine, Medical University of Vienna), allowing for transgene expression coupled to IRES-controlled GFP expression. ..

    Article Title: A systematic analysis of orphan cyclins reveals CNTD2 as a new oncogenic driver in lung cancer.
    Article Snippet: .. All the sequences were cloned into pWPI lentiviral expression vector (#12254, Addgene) at the PmeI restriction site. .. For lentivirus production, 45 μg of lentiviral expression vector were cotransfected with 12.9 μg of pMD2.G and 29.1 μg of psPAX2 into HEK293-T cells in 10 cm plates using calcium phosphate.

    Expressing:

    Article Title: CDK6 is activated by the atypical cyclin I to promote E2F-mediated gene expression and cancer cell proliferation.
    Article Snippet: .. Human cDNA of CCNI and CCND1 were amplified from cells with a C-terminal Flag tag and cloned into pWPI lentiviral expression vector (#12254; Addgene, Watertown, MA, USA). ..

    Article Title: Select human cancer mutants of NRMT1 alter its catalytic activity and decrease N-terminal trimethylation.
    Article Snippet: .. Wild type (WT), N209I, or P211S human NRMT1 were amplified from the pet15b vector to introduce a 5’ PmeI restriction site and a 3’ PmeI restriction site, and subcloned into pWPI lentiviral expression vector (Addgene). .. GFP-tagged lentivirus expressing WT NRMT1, N209I NRMT1, or P211S NRMT1 were made by co-transfecting HEK293LT cells with 50 µg pWPI containing the appropriate NRMT1 cDNA, 37.5 µg psPAX2 packaging vector, and 15 µg pMD2.G envelope plasmid using calcium phosphate transfection.

    Article Title: CDK6 is activated by the atypical cyclin I to promote E2F ‐mediated gene expression and cancer cell proliferation
    Article Snippet: .. Human cDNA of CCNI and CCND1 were amplified from cells with a C‐terminal Flag tag and cloned into pWPI lentiviral expression vector (#12254; Addgene, Watertown, MA, USA). ..

    Article Title: A systematic analysis of orphan cyclins reveals CNTD2 as a new oncogenic driver in lung cancer
    Article Snippet: .. All the sequences were cloned into pWPI lentiviral expression vector (#12254, Addgene) at the Pme I restriction site. .. For lentivirus production, 45 μg of lentiviral expression vector were cotransfected with 12.9 μg of pMD2.G and 29.1 μg of psPAX2 into HEK293-T cells in 10 cm plates using calcium phosphate.

    Article Title: A lineage-specific STAT5B N642H mouse model to study NK-cell leukemia
    Article Snippet: Parental and transduced KHYG-14 (purchased from DSMZ) and IMC-15 (kindly provided by Prof. Chen, University of New Mexico Comprehensive Cancer Center) human NK-cell lines were cultured with RPMI complete with 100 U/ml, 25 U/ml or without human IL-2 (Proleukin, SteriMax). .. Lentiviral transduction of human NK-cell lines Human non-mutant (WT) STAT5B or STAT5BN642H transgenes were cloned into a pWPI lentiviral expression vector (Addgene plasmid # 12254; kindly provided by the lab of Prof. Hoermann, previously at the Department of Laboratory Medicine, Medical University of Vienna), allowing for transgene expression coupled to IRES-controlled GFP expression. ..

    Article Title: Select human cancer mutants of NRMT1 alter its catalytic activity and decrease N‐terminal trimethylation
    Article Snippet: .. Wild type (WT), N209I, or P211S human NRMT1 were amplified from the pet15b vector to introduce a 5′ PmeI restriction site and a 3′ PmeI restriction site, and subcloned into pWPI lentiviral expression vector (Addgene). .. GFP‐tagged lentivirus expressing WT NRMT1, N209I NRMT1, or P211S NRMT1 were made by cotransfecting HEK293LT cells with 50 μg pWPI containing the appropriate NRMT1 cDNA, 37.5 μg psPAX2 packaging vector, and 15 μg pMD2.G envelope plasmid using calcium phosphate transfection.

    Article Title: N-terminal acetylation and methylation differentially affect the function of MYL9
    Article Snippet: .. To generate C-terminally tagged MYL9-FLAG proteins, WT, SPK, and SSQ MYL9 were amplified from the MYL9-pDendra2 constructs and subcloned into the pWPI lentiviral expression vector (Addgene, Cambridge, MA) using 5′ and 3′ PmeI restriction sites. .. The following 3′ primer included the FLAG nucleotide sequence followed by a stop codon: 5′-CGGTTTAAACTCATTTATCATCATCATCTTTATAATCGTCGTCTTT ATCCTTGGCGC-3′ Lentivirus was generated through co-transfecting HEK293T cells with 50 μg of expression plasmid (pCDH or pWPI from above), 37.5 μg psPAX2 packaging vector, and 15 μg pMD2.G envelope plasmid using calcium phosphate transfection as previously described [ 47 ].

    Article Title: A systematic analysis of orphan cyclins reveals CNTD2 as a new oncogenic driver in lung cancer.
    Article Snippet: .. All the sequences were cloned into pWPI lentiviral expression vector (#12254, Addgene) at the PmeI restriction site. .. For lentivirus production, 45 μg of lentiviral expression vector were cotransfected with 12.9 μg of pMD2.G and 29.1 μg of psPAX2 into HEK293-T cells in 10 cm plates using calcium phosphate.

    Introduce:

    Article Title: Select human cancer mutants of NRMT1 alter its catalytic activity and decrease N-terminal trimethylation.
    Article Snippet: .. Wild type (WT), N209I, or P211S human NRMT1 were amplified from the pet15b vector to introduce a 5’ PmeI restriction site and a 3’ PmeI restriction site, and subcloned into pWPI lentiviral expression vector (Addgene). .. GFP-tagged lentivirus expressing WT NRMT1, N209I NRMT1, or P211S NRMT1 were made by co-transfecting HEK293LT cells with 50 µg pWPI containing the appropriate NRMT1 cDNA, 37.5 µg psPAX2 packaging vector, and 15 µg pMD2.G envelope plasmid using calcium phosphate transfection.

    Article Title: Select human cancer mutants of NRMT1 alter its catalytic activity and decrease N‐terminal trimethylation
    Article Snippet: .. Wild type (WT), N209I, or P211S human NRMT1 were amplified from the pet15b vector to introduce a 5′ PmeI restriction site and a 3′ PmeI restriction site, and subcloned into pWPI lentiviral expression vector (Addgene). .. GFP‐tagged lentivirus expressing WT NRMT1, N209I NRMT1, or P211S NRMT1 were made by cotransfecting HEK293LT cells with 50 μg pWPI containing the appropriate NRMT1 cDNA, 37.5 μg psPAX2 packaging vector, and 15 μg pMD2.G envelope plasmid using calcium phosphate transfection.

    Plasmid Preparation:

    Article Title: Select human cancer mutants of NRMT1 alter its catalytic activity and decrease N-terminal trimethylation.
    Article Snippet: .. Wild type (WT), N209I, or P211S human NRMT1 were amplified from the pet15b vector to introduce a 5’ PmeI restriction site and a 3’ PmeI restriction site, and subcloned into pWPI lentiviral expression vector (Addgene). .. GFP-tagged lentivirus expressing WT NRMT1, N209I NRMT1, or P211S NRMT1 were made by co-transfecting HEK293LT cells with 50 µg pWPI containing the appropriate NRMT1 cDNA, 37.5 µg psPAX2 packaging vector, and 15 µg pMD2.G envelope plasmid using calcium phosphate transfection.

    Article Title: A systematic analysis of orphan cyclins reveals CNTD2 as a new oncogenic driver in lung cancer
    Article Snippet: .. All the sequences were cloned into pWPI lentiviral expression vector (#12254, Addgene) at the Pme I restriction site. .. For lentivirus production, 45 μg of lentiviral expression vector were cotransfected with 12.9 μg of pMD2.G and 29.1 μg of psPAX2 into HEK293-T cells in 10 cm plates using calcium phosphate.

    Article Title: A lineage-specific STAT5B N642H mouse model to study NK-cell leukemia
    Article Snippet: Parental and transduced KHYG-14 (purchased from DSMZ) and IMC-15 (kindly provided by Prof. Chen, University of New Mexico Comprehensive Cancer Center) human NK-cell lines were cultured with RPMI complete with 100 U/ml, 25 U/ml or without human IL-2 (Proleukin, SteriMax). .. Lentiviral transduction of human NK-cell lines Human non-mutant (WT) STAT5B or STAT5BN642H transgenes were cloned into a pWPI lentiviral expression vector (Addgene plasmid # 12254; kindly provided by the lab of Prof. Hoermann, previously at the Department of Laboratory Medicine, Medical University of Vienna), allowing for transgene expression coupled to IRES-controlled GFP expression. ..

    Article Title: Select human cancer mutants of NRMT1 alter its catalytic activity and decrease N‐terminal trimethylation
    Article Snippet: .. Wild type (WT), N209I, or P211S human NRMT1 were amplified from the pet15b vector to introduce a 5′ PmeI restriction site and a 3′ PmeI restriction site, and subcloned into pWPI lentiviral expression vector (Addgene). .. GFP‐tagged lentivirus expressing WT NRMT1, N209I NRMT1, or P211S NRMT1 were made by cotransfecting HEK293LT cells with 50 μg pWPI containing the appropriate NRMT1 cDNA, 37.5 μg psPAX2 packaging vector, and 15 μg pMD2.G envelope plasmid using calcium phosphate transfection.

    Article Title: A systematic analysis of orphan cyclins reveals CNTD2 as a new oncogenic driver in lung cancer.
    Article Snippet: .. All the sequences were cloned into pWPI lentiviral expression vector (#12254, Addgene) at the PmeI restriction site. .. For lentivirus production, 45 μg of lentiviral expression vector were cotransfected with 12.9 μg of pMD2.G and 29.1 μg of psPAX2 into HEK293-T cells in 10 cm plates using calcium phosphate.

    Transduction:

    Article Title: A lineage-specific STAT5B N642H mouse model to study NK-cell leukemia
    Article Snippet: Parental and transduced KHYG-14 (purchased from DSMZ) and IMC-15 (kindly provided by Prof. Chen, University of New Mexico Comprehensive Cancer Center) human NK-cell lines were cultured with RPMI complete with 100 U/ml, 25 U/ml or without human IL-2 (Proleukin, SteriMax). .. Lentiviral transduction of human NK-cell lines Human non-mutant (WT) STAT5B or STAT5BN642H transgenes were cloned into a pWPI lentiviral expression vector (Addgene plasmid # 12254; kindly provided by the lab of Prof. Hoermann, previously at the Department of Laboratory Medicine, Medical University of Vienna), allowing for transgene expression coupled to IRES-controlled GFP expression. ..

    Construct:

    Article Title: N-terminal acetylation and methylation differentially affect the function of MYL9
    Article Snippet: .. To generate C-terminally tagged MYL9-FLAG proteins, WT, SPK, and SSQ MYL9 were amplified from the MYL9-pDendra2 constructs and subcloned into the pWPI lentiviral expression vector (Addgene, Cambridge, MA) using 5′ and 3′ PmeI restriction sites. .. The following 3′ primer included the FLAG nucleotide sequence followed by a stop codon: 5′-CGGTTTAAACTCATTTATCATCATCATCTTTATAATCGTCGTCTTT ATCCTTGGCGC-3′ Lentivirus was generated through co-transfecting HEK293T cells with 50 μg of expression plasmid (pCDH or pWPI from above), 37.5 μg psPAX2 packaging vector, and 15 μg pMD2.G envelope plasmid using calcium phosphate transfection as previously described [ 47 ].



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